Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Taurodeoxycholate increases intestinal epithelial cell proliferation through c-myc expression.

Jon Yamaguchi1, Alex Toledo, Barbara L Bass

  • 1Department of Surgery, University of Maryland, Baltimore, Md 21201, USA.

Surgery
|January 24, 2004
PubMed
Summary

The bile salt taurodeoxycholate significantly increases intestinal epithelial cell proliferation by promoting cell cycle progression. This effect is mediated by the c-myc pathway, suggesting a beneficial role for bile salts in intestinal health.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction: Modulation by miR-29b of intestinal epithelium homoeostasis through the repression of menin translation.

The Biochemical journal·2026
Same author

Correction: Post-transcriptional regulation of MEK-1 by polyamines through the RNA-binding protein HuR modulating intestinal epithelial apoptosis.

The Biochemical journal·2026
Same author

Correction: Induced TRPC1 expression sensitizes intestinal epithelial cells to apoptosis by inhibiting NF-κB activation through Ca2+ influx.

The Biochemical journal·2026
Same author

Fixing a Mismatch: The Case for Age-Aligned Kidney Allocation.

Transplant international : official journal of the European Society for Organ Transplantation·2025
Same author

Early surgical intervention for extensive nontuberculous mycobacterial pulmonary disease.

IJTLD open·2025
Same author

The Unmet Need for Solid Organ Transplantation in the US Pacific Islands: A Summary of Current Data and Challenges.

Transplantation·2025

Area of Science:

  • Gastroenterology
  • Cell Biology
  • Molecular Biology

Background:

  • Bile salts influence gastrointestinal epithelial functions like restitution and differentiation.
  • Taurodeoxycholate, a specific bile salt, has been previously linked to increased cell migration post-injury.

Purpose of the Study:

  • To investigate the impact of taurodeoxycholate on intestinal epithelial cell growth.
  • To examine the effects of taurodeoxycholate on c-myc expression and function.

Main Methods:

  • Intestinal epithelial cells (IEC-6, Caco-2) were treated with varying taurodeoxycholate concentrations.
  • Proliferation, apoptosis, cell cycle phase, and c-myc expression (mRNA/protein) were assessed.
  • C-myc function was inhibited using antisense oligonucleotides.

Related Experiment Videos

Main Results:

  • Taurodeoxycholate significantly increased IEC-6 cell proliferation without inducing apoptosis or altering cell structure.
  • Flow cytometry revealed a shift towards S-phase and a decrease in G1-phase of the cell cycle.
  • Increased c-myc protein and mRNA expression was observed, and its inhibition blocked taurodeoxycholate-induced proliferation.

Conclusions:

  • Physiological concentrations of taurodeoxycholate enhance intestinal epithelial cell proliferation.
  • The proliferative effect is, in part, dependent on the c-myc signaling pathway.
  • Bile salts may exert beneficial effects on the intestinal mucosa.