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[Expression of osteoclast stimulating and differentiating factors in a murine model of localized inflammatory bone
H Sudhoff1, Y Liebehenz, J Aschenbrenner
1Hals-Nasen-Ohrenklinik der Ruhr-Universität Bochum, St. Elisabeth Hospital, Bochum, Germany. Holger.Sudhoff@ruhr-uni-bochum.de
Background:
The pathology associated to cholesteatoma is predominantly a consequence of osteoclast-mediated bone resorption within the middle ear. To assess its pathogenesis a murine model for dermal-implant induced osteolysis was evaluated for the expression of osteoclast stimulating and differentiating factors.
Methods:
Mouse calvaria were analysed for the expression of osteoprotegerin ligand (OPGL), osteoprotegerin (OPG) and macrophage-colony stimulating factor (M-CSF) using immunohistochemistry. The detection of osteoclast cell lineage was acquired by immunohistochemistry using markers CD 4, CD 11a, CD 11b, CD 14, CD 51, CD 68 and TRAP.
Results:
An increased expression of the investigated cytokines M-CSF, OPG and OPGL was demonstrated by immunohistochemistry. The presence of osteoclast precursor cells and mature resorbing osteoclasts was confirmed in time-dependent manner triggered by dermal implantation.
Conclusions:
This study reveals the basic events in osteoclast biology in localized inflammatory bone resorption and provides new insights into the comprehension of cholesteatoma-induced bone resorption.
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