Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Web-based tools for mining the NCI databases for anticancer drug discovery.

Xueliang Fang1, Lei Shao, Hui Zhang

  • 1University of Michigan Comprehensive Cancer Center, Department of Internal Medicine, University of Michigan, 1500 E Medical Center Drive, Ann Arbor, Michigan 48109-0934, USA.

Journal of Chemical Information and Computer Sciences
|January 27, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibitory crosstalk between ERK and AMPK in the growth and proliferation of cardiac fibroblasts.

Biochemical and biophysical research communications·2008
Same author

mTORC1 signaling requires proteasomal function and the involvement of CUL4-DDB1 ubiquitin E3 ligase.

Cell cycle (Georgetown, Tex.)·2008
Same author

Prospective study of liver transplant recipients with HCV infection: evidence for a causal relationship between HCV and insulin resistance.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·2008
Same author

Quantitative gel electrophoresis: sources of variation.

Journal of proteome research·2008
Same author

Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a process similar to, but distinct from, cellular DSB repair.

Virology journal·2008
Same author

[Inhibitory effects of Qushi Huayu Decoction on fatty deposition and tumor necrosis factor alpha secretion in HepG2 cells induced by free fatty acid].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2008

This study introduces web-based tools for cancer drug discovery using National Cancer Institute (NCI) databases. These tools analyze drug activity and gene expression to identify potential drug leads and mechanisms.

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Drug Discovery

Background:

  • The National Cancer Institute (NCI) maintains extensive anticancer databases crucial for drug discovery.
  • Identifying novel anticancer compounds and understanding their mechanisms requires sophisticated data mining approaches.

Purpose of the Study:

  • To develop and present integrated Web-based tools for mining NCI anticancer databases.
  • To facilitate the identification of potential anticancer drug leads and elucidate drug mechanisms of action.

Main Methods:

  • Implementation of three correlation algorithms: Pearson's, Spearman's, and Kendall's.
  • Integration of p-value testing to assess the statistical significance of correlation analyses.
  • Development of a Web-based platform for analyzing in vitro anticancer activity against NCI 60 cell line molecular data (protein and mRNA levels).

Related Experiment Videos

Main Results:

  • The developed tools enable robust correlation analysis between drug activity and molecular target expression.
  • Successfully identified potential protein kinase C (PKC) ligands using a lead compound approach.
  • Identified potential ErbB-2 inhibitors by analyzing ErbB-2 mRNA levels in NCI 60 cancer cell lines.

Conclusions:

  • The integrated Web-based tools provide a powerful platform for anticancer drug discovery and mechanism studies.
  • These tools enhance the ability to correlate in vitro drug activity with molecular targets for lead compound identification.
  • The methodology supports the systematic exploration of NCI databases for novel therapeutic strategies.