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Simultaneous determination of retinol and tocopherols by high-performance liquid chromatography
Y Satomura1, M Kimura, Y Itokawa
1Department of Hygiene, Faculty of Medicine, Kyoto University, Japan.
Journal of Chromatography
|November 20, 1992
Abstract:
A high-performance liquid chromatographic (HPLC) method to determine retinol and all four tocopherols (alpha-, beta-, gamma- and delta-) simultaneously was established using a reversed-phase column (YMC-PACK A-302 S-5 120A ODS). The HPLC conditions were mobile phase 65% isopropanol, sample solvent 99.5% methanol and temperature 30 degrees C. Retinol and tocopherols were measured in rat liver.