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Updated: Aug 29, 2026
Isolation, Transfection, and Culture of Primary Human Monocytes
Published on: December 16, 2019
Proteomic fingerprinting of HIV-1-infected human monocyte-derived macrophages: a preliminary report
Kimberly A Carlson1, Pawel Ciborowski, Courtney N Schellpeper
1Center for Neurovirology and Neurodegenerative Disorders, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5215, USA.
Abstract:
Mononuclear phagocytes (MP; blood monocytes, alveolar, lymph node, and brain macrophages and microglia) are vehicles for dissemination and principle target cells for human immunodeficiency virus type 1 (HIV-1) infection. Notably, viral persistence in macrophages occurs despite ongoing phagocytic, intracellular killing, innate and adaptive immune responses. To assess potential pathways for how HIV-1 may bypass antiviral MP responses, we used proteomic tests to evaluate protein fingerprints of HIV-1-infected human monocyte-derived macrophages 7 days after viral infection. By using weak cation exchange chips, 58 proteins were found up- or down-regulated after HIV-1(ADA) infection. Several of these proteins were identified by microsequencing. It is probable that cellular proteins identified by proteomic fingerprinting could assist in unraveling how persistent viral infection occurs in MP lineage cells. Moreover, this evolving technology can be utilized to unravel changes in immune activities initiated by interactions between virus, environmental cues and drugs of abuse.
Insights
Human immunodeficiency virus type 1 (HIV-1) persists in mononuclear phagocytes (MP) by altering cellular proteins. Proteomic analysis identified 58 differentially expressed proteins, offering insights into viral persistence mechanisms.
Area of Science:
- Virology
- Immunology
- Proteomics
Background:
- Mononuclear phagocytes (MP) are key targets for human immunodeficiency virus type 1 (HIV-1).
- HIV-1 establishes persistent infections in MP despite robust immune responses.
Purpose of the Study:
- To investigate the molecular mechanisms by which HIV-1 persists within MP.
- To identify host cell proteins altered during HIV-1 infection in MP.
Main Methods:
- Proteomic analysis using weak cation exchange chips.
- Cultured human monocyte-derived macrophages infected with HIV-1(ADA).
- Protein identification via microsequencing.
Main Results:
- HIV-1 infection resulted in the up- or down-regulation of 58 proteins in macrophages.
- Several differentially expressed proteins were identified.
Conclusions:
- Proteomic profiling reveals host protein changes associated with persistent HIV-1 infection in MP.
- These identified proteins may elucidate pathways for viral persistence and inform therapeutic strategies.
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