Soluble phosphate glasses: in vitro studies using human cells of hard and soft tissue origin

This report describes the short-term response of two typical cellular components of a hard/soft tissue interface such as the periodontal ligament/mandible and patellar tendon/tibia. Tissue engineering of such interfaces requires a contiguous scaffold system with at least two cell types associated with it. Human oral osteoblasts, oral fibroblasts and hand flexor tendon fibroblasts were seeded on phosphate-based glasses of different dissolution rates. Quantitative and morphological assessment of cell adhesion and proliferation for all cell types was assessed, after first elucidating an experimental composition range using MG63 cells. In addition, immunolabelling of bone-specific non-collagenous proteins bone sialoprotein, osteonectin and osteopontin was performed to determine osteoblast phenotype. Fibroblast phenotype was established by immunolabelling for prolyl-4-hydroxylase, an enzyme vital for collagen biosynthesis. Results indicated that both cell types maintained their respective phenotypes over time in culture on glass discs of generic composition (CaO)x-(Na2O)(0.5-x)-(P2O5)0.5, remained attached and proliferated dependent on glass composition and cell type. Glasses containing at least 46 mol% CaO, produced no adverse cell reaction suggesting that these compositions that support both osteoblasts and fibroblasts would be ideal as a scaffold material for engineering the hard/soft tissue interface.