Lactobacillus paracollinoides sp. nov., isolated from brewery environments

Three novel strains isolated from brewery environments are described. These strains were Gram-positive, facultatively anaerobic, heterofermentative rods that did not exhibit catalase activity. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that these strains belong to the genus Lactobacillus and are most closely related to Lactobacillus collinoides (approximately 99% similarity). The novel strains could be differentiated from L. collinoides on the basis of DNA-DNA relatedness, differences in beer-spoilage ability and the inability to utilize D-fructose. These isolates represent a novel species, for which the name Lactobacillus paracollinoides sp. nov. is proposed. The type strain is LA2T (=DSM 15502T=JCM 11969T).