Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kalirin expression is regulated by multiple promoters.

Clifton E McPherson1, Betty A Eipper, Richard E Mains

  • 1Department of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030, USA.

Journal of Molecular Neuroscience : MN
|January 27, 2004
PubMed
Summary

Kalirin, a guanine nucleotide exchange factor, exhibits complex expression patterns due to multiple transcriptional start sites and alternative first exons. These variations influence protein isoforms and their roles in brain development across species.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tissue Damage in Rheumatoid Arthritis Is Genetically Linked to Low Peptidylglycine Alpha-Amidating Monooxygenase Activity in Synovial Fibroblasts.

Arthritis & rheumatology (Hoboken, N.J.)·2026
Same author

Peptidylglycine alpha-amidating monooxygenase is important in mice for beta-cell cilia formation and insulin secretion but promotes diabetes risk through beta-cell independent mechanisms.

Molecular metabolism·2025
Same author

Germline loss-of-function <i>PAM</i> variants are enriched in subjects with pituitary hypersecretion.

Frontiers in endocrinology·2023
Same author

Germline loss-of-function <i>PAM</i> variants are enriched in subjects with pituitary hypersecretion.

medRxiv : the preprint server for health sciences·2023
Same author

Structure of the Sec14 domain of Kalirin reveals a distinct class of lipid-binding module in RhoGEFs.

Nature communications·2023
Same author

Regulated processing and secretion of a peptide precursor in cilia.

Proceedings of the National Academy of Sciences of the United States of America·2022

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Kalirin is a guanine nucleotide exchange factor (GEF) crucial for Rho family GTPases.
  • It is known to exist in multiple isoforms with varied functional domains.
  • Kalirin expression patterns are complex, particularly during brain development.

Purpose of the Study:

  • To investigate the molecular basis of Kalirin's complex expression.
  • To identify and characterize alternative transcriptional start sites and their impact on Kalirin isoforms.
  • To compare Kalirin gene regulation in rats and humans.

Main Methods:

  • Analysis of Kalirin transcripts in rat and human samples.
  • Identification of multiple transcriptional start sites and alternative first exons.

Related Experiment Videos

  • Comparative analysis of Kalirin promoter regions in rats and humans.

    • Main Results:

    • Multiple transcriptional start sites generate Kalirin transcripts with distinct 5' ends and amino termini.
    • Alternative first exons exhibit differential expression patterns in developing brains and cell cultures.
    • These alternative exons are located significantly upstream of exon 2 (>100 kb).
    • Rat and human Kalirin promoter regions share numerous potential regulatory elements.

    Conclusions:

    • Kalirin gene expression is regulated by alternative transcriptional initiation, leading to diverse protein isoforms.
    • These regulatory mechanisms contribute to the complex expression patterns observed during brain development.
    • Conserved regulatory elements in rat and human promoters suggest shared mechanisms of Kalirin gene control.