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Updated: Aug 6, 2026

Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
Published on: August 3, 2009
Ongoing development of two-dimensional polyacrylamide gel electrophoresis data standards
Veerasamy Ravichandran1, Joshua Lubell, Gregory B Vásquez
1Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD, USA. vravi@nist.gov
Abstract:
We present an approach toward standardizing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) data in support of developing a globally relevant proteomics consensus in order to provide more efficient database querying and data comparisons through the establishment of the necessary definitions and interdisciplinary reference fields for both the 2-D PAGE community, particularly in the proteomics area, and the clinical and experimental biological research communities, in general. This article covers the need for unifying the 2-D PAGE data through a common data repository, and its usefulness in data standards and data interoperability.
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