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The Kazusa cDNA project for identification of unknown human transcripts
Takahiro Nagase1, Reiko Kikuno, Osamu Ohara
1Department of Human Gene Research, Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.
Comptes Rendus Biologies
|January 28, 2004
Summary
The Kazusa cDNA Project sequenced over 2000 large human complementary DNAs (cDNAs), integrating this data with the human genome sequence. Future work will refine these clones to bridge the human genome and proteome.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- The Kazusa cDNA Project focuses on sequencing large human complementary DNAs (cDNAs), exceeding 4 kilobases.
- Understanding the human genome and proteome requires comprehensive cDNA sequence data.
Purpose of the Study:
- To provide an overview of human cDNA sequence data from the first phase of the Kazusa cDNA Project.
- To integrate accumulated cDNA data with the human genome sequence.
- To prepare for the second phase, aiming to connect the human genome and proteome.
Main Methods:
- Sequencing of over 2000 large human cDNAs (>4 kb).
- Integration of cDNA sequence data with existing human genome sequence data.
- Careful evaluation and experimental revision of cDNA clones for the second phase.
Main Results:
- Accumulation of human cDNA sequence data for over 2000 clones.
- Successful integration of this cDNA data with the human genome sequence.
- Establishment of a foundation for bridging the human genome and proteome.
Conclusions:
- The first phase of the Kazusa cDNA Project has generated a significant dataset of large human cDNAs.
- This data is integrated with the human genome, providing a valuable resource.
- Ongoing efforts focus on refining cDNA clones to enhance the understanding of the human genome-proteome relationship.