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YY1AP, a novel co-activator of YY1

Chen-Yu Wang1, Yuh-Jin Liang, Young-Sun Lin

  • 1Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan.

The Journal of Biological Chemistry
|January 28, 2004
PubMed

Insights

Researchers identified YY1AP, a novel protein that interacts with YY1 (Yin Yang 1) to enhance gene transcription. This discovery sheds light on the regulation of YY1 activity in human cells.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Protein-Protein Interactions

Background:

  • The transcription factor YY1 (Yin Yang 1) plays a crucial role in regulating gene expression.
  • Identifying proteins that interact with YY1 is essential for understanding its regulatory mechanisms.

Purpose of the Study:

  • To identify novel cellular proteins that mediate the transcriptional regulation of YY1.
  • To characterize the function and properties of a newly identified YY1-interacting protein.

Main Methods:

  • Yeast two-hybrid screening of a HeLa cDNA library to identify YY1-interacting proteins.
  • 5'-rapid amplification of cDNA ends (5'-RACE) for full-length cDNA cloning and sequencing.
  • Northern blot analysis for gene expression profiling.
  • Co-immunoprecipitation and immunostaining for subcellular localization.
  • Glutathione S-transferase (GST) pull-down assays for interaction mapping.

Main Results:

  • A novel protein, YY1AP, was identified and found to interact with YY1.
  • YY1AP cDNA is 2253 bp, encoding a 750-amino acid protein, located on chromosome 1 (1q21.3).
  • YY1AP is ubiquitously expressed and co-localizes with YY1 in the nucleus.
  • YY1AP enhances YY1-responsive promoter activity and contains two YY1 binding regions.

Conclusions:

  • YY1AP is a novel co-activator of YY1.
  • YY1AP plays a significant role in modulating YY1-mediated transcriptional regulation.
  • Further research into YY1AP could reveal new therapeutic targets for diseases involving YY1 dysregulation.

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