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Related Experiment Videos

Posterior vitreous detachment induced by microplasmin.

Arnd Gandorfer1, Matthias Rohleder, Charanjit Sethi

  • 1Department of Ophthalmology, University Eye Hospital, Ludwig-Maximilians-University, Munich, Germany. arnd.gandorfer@ak-i.med.uni-muenchen.de

Investigative Ophthalmology & Visual Science
|January 28, 2004
PubMed
Summary

Microplasmin effectively induces posterior vitreous detachment (PVD) by cleaving the vitreous cortex from the retina. This method preserves retinal integrity in both human and feline eyes.

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Area of Science:

  • Ophthalmology
  • Retinal Science
  • Vitreoretinal Surgery

Background:

  • Posterior vitreous detachment (PVD) is a common age-related condition.
  • Inducing PVD can be beneficial for treating certain retinal diseases.
  • Current methods for PVD induction have limitations.

Purpose of the Study:

  • To assess the efficacy of microplasmin in inducing PVD.
  • To evaluate the safety of microplasmin on the retina in human and feline models.
  • To understand the dose-dependent effects of microplasmin on vitreous-retinal adhesion.

Main Methods:

  • Human donor eyes and feline eyes were treated with varying doses of microplasmin.
  • Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used for analysis.

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  • Retinal specimens were examined after intravitreal injection and incubation periods.

    • Main Results:

    • Microplasmin induced complete PVD at doses of 125 and 188 micro g in human eyes.
    • In vivo studies in cats showed complete PVD with 25 micro g microplasmin after 3 days.
    • No morphologic alterations of the retina or cellular response were observed in any treated eyes.

    Conclusions:

    • Microplasmin effectively induces dose-dependent PVD in postmortem and in vivo models.
    • The treatment preserves retinal structure and does not elicit cellular responses in glial cells or neurons.
    • Microplasmin presents a promising agent for controlled PVD induction in ophthalmology.