Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multi-dimensional pulsed field gradient magic angle spinning NMR experiments on membranes.

Holly C Gaede1, Klaus Gawrisch

  • 1Laboratory of Membrane Biochemistry and Biophysics, NIAAA, NIH, 12420 Parklawn Drive, Rockville, Maryland 20852, USA.

Magnetic Resonance in Chemistry : MRC
|January 28, 2004
PubMed
Summary

Gradient Nuclear Magnetic Resonance (NMR) techniques enhance the study of lipid membranes, revealing ibuprofen

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Small angle neutron scattering study of rhodopsin oligomerization and G-protein coupling in a physiologically relevant lipid membrane.

Biochimica et biophysica acta. Biomembranes·2025
Same author

Solid-state NMR of membrane peptides and proteins in the lipid cubic phase.

Biophysical journal·2025
Same author

Probing the Conformational Space of the Cannabinoid Receptor 2 and a Systematic Investigation of DNP-Enhanced MAS NMR Spectroscopy of Proteins in Detergent Micelles.

ACS omega·2023
Same author

Physiological changes in bilayer thickness induced by cholesterol control GPCR rhodopsin function.

Biophysical journal·2022
Same author

Detection of cannabinoid receptor type 2 in native cells and zebrafish with a highly potent, cell-permeable fluorescent probe.

Chemical science·2022
Same author

Site-selective labeling and electron paramagnetic resonance studies of human cannabinoid receptor CB<sub>2</sub>.

Biochimica et biophysica acta. Biomembranes·2021

Area of Science:

  • Biophysical Chemistry
  • Membrane Biophysics
  • Nuclear Magnetic Resonance Spectroscopy

Background:

  • Lipid membranes are complex systems crucial for biological processes.
  • Understanding drug-membrane interactions, like ibuprofen in liposomes, requires advanced analytical methods.
  • Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful tool for molecular studies.

Purpose of the Study:

  • To demonstrate the benefits of gradient techniques in studying lipid membranes.
  • To investigate the behavior of ibuprofen within 1-palmitoyl-2-oleoyl-sn-glycero-3 phosphocholine (POPC) liposomes.
  • To present a theoretical framework for analyzing diffusion in multilamellar liposomes using magic angle spinning (MAS).

Main Methods:

  • Application of gradient-enhanced homo- and heteronuclear chemical shift correlation NMR.

Related Experiment Videos

  • Utilizing gradient NOESY experiments for structural and dynamic insights.
  • Performing diffusion measurements with pulsed field gradients under MAS conditions.

    • Main Results:

    • Successful resonance assignments were achieved using gradient-enhanced NMR techniques.
    • Gradient NOESY experiments provided insights into the location and dynamics of lipids, ibuprofen, and water.
    • Diffusion measurements characterized the lateral mobility of lipid and drug molecules within the membrane.

    Conclusions:

    • Gradient NMR techniques are highly effective for analyzing lipid membrane systems, including drug-membrane interactions.
    • These methods accelerate experiments and improve spectral quality by reducing noise.
    • The study provides a foundation for analyzing molecular diffusion in complex membrane systems.