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Related Experiment Videos

Early requirement for fgf8 function during hindbrain pattern formation in zebrafish.

Elizabeth L Wiellette1, Hazel Sive

  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|January 28, 2004
PubMed
Summary

Fibroblast growth factor 8 (FGF8) is crucial for early hindbrain development in zebrafish. FGF8 alone initiates rhombomere 5 and 6 development, with FGF3 supporting later stages.

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Area of Science:

  • Developmental biology
  • Neuroscience
  • Genetics

Background:

  • Fibroblast growth factor (FGF) signaling plays a vital role in vertebrate brain development.
  • Previous studies in zebrafish highlighted the combined roles of FGF3 and FGF8 in specifying hindbrain rhombomeres 5 and 6 (r5, r6) during mid- to late somitogenesis.
  • The precise role of FGF signaling during early somitogenesis remained less understood.

Purpose of the Study:

  • To investigate the specific requirement of FGF signaling during early somitogenesis for hindbrain patterning.
  • To determine the independent roles of FGF3 and FGF8 in the initial specification of r5 and r6.
  • To elucidate the mechanisms underlying the recovery of gene expression in r5 and r6 during later developmental stages.

Main Methods:

  • Utilized zebrafish as a model organism.

Related Experiment Videos

  • Employed genetic mutations in fgf8.
  • Administered fgf8-targeted antisense morpholino oligonucleotides to inhibit FGF8 function.
  • Monitored gene expression patterns in r5 and r6 during early and late somitogenesis stages.

    • Main Results:

    • FGF8 signaling alone is essential for initiating r5 and r6 development during early somitogenesis.
    • Disruption of fgf8 function led to the suppression of key genes in r5 and r6 by the one- to two-somite stage.
    • Gene expression in r5 and r6 recovered by the six-somite stage.
    • This recovery is proposed to be mediated by the activation of fgf3 and delayed FGF8 accumulation.

    Conclusions:

    • Demonstrates an early, nonredundant requirement for FGF8 in hindbrain patterning.
    • Highlights the distinct temporal roles of FGF8 and FGF3 in specifying hindbrain segments.
    • Provides insights into the compensatory mechanisms regulating FGF signaling during embryonic development.