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Novel method for cell immobilization and its application for production of oligosaccharides from sucrose
1Faculty of Life Science and Technology, Sungkyunkwan University, Suwon, Kyunggi-do, Korea.
Aims:
The purpose of the present investigation was to develop a novel method for cell immobilization.
Methods And Results:
Aureobasidium pullulans cells were mixed with an alginate solution, and the mixture was extruded to form small gel beads as hydrated-immobilized cells. The beads were then placed at -15 degrees C for 6-24 h to induce freeze-dehydration. The freeze-dehydration resulted in shrinkage of beads as a result of water removal reducing bead volume by 82% and bead weight by 85%. The dehydrated beads were successfully used for the production of fructo-oligosaccharides in a model reactor system.
Conclusions:
Dehydrated beads may provide some commercial advantages over conventional immobilized cells.
Significance And Impact Of The Study:
This study shows that bioreactor performance can be improved up to two times by the use of the dehydrated beads.
