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Updated: Aug 29, 2026
Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
Published on: November 16, 2011
Comparison of the [13C]glucose breath test to the hyperinsulinemic-euglycemic clamp when determining insulin
Richard Z Lewanczuk1, Breay W Paty, Ellen L Toth
1Division of Endocrinology, University of Alberta, Edmonton, Canada. rlewancz@gpu.srv.ualberta.ca
Objective:
With increasing emphasis on the recognition of the metabolic syndrome and early type 2 diabetes, a clinically useful measure of insulin resistance is desirable. The purpose of this study was to evaluate whether an index of glucose metabolism, as measured by (13)CO(2) generation from ingested [(13)C]glucose, would correlate with indexes from the hyperinsulinemic-euglycemic clamp.
Research Design And Methods:
A total of 26 subjects with varying degrees of insulin sensitivity underwent both the [(13)C]glucose breath test and the hyperinsulinemic-euglycemic clamp. Results from the [(13)C]glucose breath test were compared with measures of insulin sensitivity from the glucose clamp as well as with other commonly used indexes of insulin sensitivity.
Results:
There was a strong correlation between the [(13)C]glucose breath test result and the glucose disposal rate (r = 0.69, P < 0.0001) and insulin sensitivity index (r = 0.69, P < 0.0001) from the insulin clamp. The magnitude of these correlations compared favorably with QUICKI and were superior to the homeostasis model assessment.
Conclusions:
The [(13)C]glucose breath test may provide a useful noninvasive assessment of insulin sensitivity.
