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No association between oxidative stress and IL-6 in NIDDM patients with nephropathy
S Alsancak1, M Aydin, A Yildiz
1Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, Istanbul, Turkey.
Abstract:
Oxidative stress is hypothesized to play a role in the development of diabetes with and without nephropathy. In addition, it has been suggested that some metabolic abormalities associated with diabetes may be due to cytokine overproduction. In the light of this knowledge, we aimed to measure MDA levels as a marker of oxidative stress and the IL-6 level in diabetes with and without different stages of nephropathy. Plasma MDA levels in the group of NIDDM patients with advanced nephropathy were significantly higher than in the group of NIDDM patients without nephropathy, which had significantly higher levels compared with the control group. Although IL-6 levels were elevated in diabetic groups with and without nephropathy in comparison with the control, no significant difference was found between patient groups. As a conclusion, oxidative stress may play an important role in diabetes with and without nephropathy, but the IL-6 level may not be useful in the evaluation of diabetic nephropathy.
Insights
Oxidative stress, indicated by malondialdehyde (MDA) levels, is elevated in diabetes with nephropathy. Interleukin-6 (IL-6) levels were not significantly different between diabetic patient groups, suggesting it may not be a useful marker for diabetic nephropathy.
Area of Science:
- Endocrinology and Metabolism
- Nephrology
- Biochemistry
Background:
- Oxidative stress is implicated in the pathogenesis of diabetes and its complications, including nephropathy.
- Cytokine overproduction has been proposed as a contributor to metabolic abnormalities in diabetes.
Purpose of the Study:
- To investigate the role of oxidative stress and inflammation in non-insulin-dependent diabetes mellitus (NIDDM) with varying stages of nephropathy.
- To measure plasma malondialdehyde (MDA) as a marker of oxidative stress and interleukin-6 (IL-6) levels in diabetic patients.
Main Methods:
- Plasma MDA and IL-6 levels were measured in three groups: healthy controls, NIDDM patients without nephropathy, and NIDDM patients with advanced nephropathy.
- Statistical analysis was performed to compare marker levels across the groups.
Main Results:
- Plasma MDA levels were significantly higher in NIDDM patients with advanced nephropathy compared to those without nephropathy.
- MDA levels were also significantly higher in NIDDM patients without nephropathy compared to the control group.
- While IL-6 levels were elevated in diabetic groups compared to controls, no significant differences were observed between the diabetic patient groups.
Conclusions:
- Oxidative stress, as indicated by elevated MDA, appears to play a significant role in the development and progression of diabetic nephropathy.
- Interleukin-6 (IL-6) may not be a reliable biomarker for evaluating the presence or severity of diabetic nephropathy.