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The human parainfluenza virus type 3 (HPIV 3) C protein inhibits viral transcription

Achut G Malur1, Michael A Hoffman, Amiya K Banerjee

  • 1Department of Virology NN-10, The Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.

Virus Research
|January 30, 2004
PubMed

Insights

The human parainfluenza virus type 3 (HPIV 3) C protein inhibits viral transcription. This inhibition is dose-dependent and may involve protein oligomerization, as shown by a coiled-coil motif deletion.

Area of Science:

  • Virology
  • Molecular Biology

Background:

  • Human parainfluenza virus type 3 (HPIV 3) C protein is synthesized from an alternate open reading frame within the phosphoprotein (P) mRNA.
  • The specific roles of HPIV 3 C, D, and V proteins in viral transcription and replication are not fully understood.

Purpose of the Study:

  • To investigate the role of the HPIV 3 C protein in viral transcription.
  • To determine if the C protein's function involves interactions with other viral components.

Main Methods:

  • Utilized an HPIV 3 minigenome construct to monitor luciferase reporter gene expression.
  • Assessed the effect of the HPIV 3 C protein on transcription in a dose-dependent manner.
  • Examined heterologous interactions using the Sendai virus (Se-V) C protein and analyzed a coiled-coil motif deletion mutant.

Main Results:

  • HPIV 3 C protein significantly inhibits minigenome transcription in a dose-dependent manner.
  • Sendai virus C protein also inhibited HPIV 3 minigenome transcription, indicating a heterologous interaction.
  • Deletion of a coiled-coil motif in the C protein abrogated its inhibitory effect, suggesting oligomerization is involved.

Conclusions:

  • The HPIV 3 C protein plays an inhibitory role in viral transcription.
  • Oligomerization of the C protein, potentially mediated by its coiled-coil motif, is implicated in the inhibition of transcription.

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