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Updated: Jul 17, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Published on: August 27, 2014
The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures
P O Vardevanyan1, A P Antonyan, M A Parsadanyan
1Biophysics Department of the Biological, Faculty of Yerevan State University, 375025 Yerevan, Aleck Manoogian St. 1, Armenia.
Abstract:
The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding isotherms of EtBr on double and single-stranded DNA at pH = 7.0 and pH = 3.0 (t = 25(o)C) are obtained by absorption and fluorimetric methods. Binding constants (K) and number of bases (n), corresponding to one binding site were determined. Non fluorescent "strong" complex with ds-DNA at pH = 7.0 and t = 25(o)C as well as "strong" and "weak" complexes with ss-DNA at pH = 3.0 and t = 25(o)C are revealed.
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