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Delta sleep-inducing peptide and its tetrapeptide analogue alleviate severity of metaphit seizures
Olivera Stanojlović1, Dragana Zivanović, Slobodan Mirković
1Department of Physiology, School of Medicine, University of Belgrade, Visegradska 26/II, 11000, Serbia, Belgrade, Yugoslavia. maxol@eunet.yu
Abstract:
The effects of delta sleep-inducing peptide (DSIP) and its tetrapeptide analogue, DSIP(1-4), on metaphit-induced audiogenic seizures were studied. Five groups of adult male Wistar rats were intraperitoneally treated with (1) saline, (2) metaphit, (3) DSIP, (4) metaphit+DSIP and (5) metaphit+DSIP(1-4). To examine blocking effects of DSIP and its analogue on fully developed metaphit seizures, the last two groups were injected after the eight audiogenic testing. The rats were stimulated using electric bell (on the top of the cage, generating 100+/-3 dB and frequency 5-8 kHz, for 60 s) 1 h after metaphit and afterwards at hourly intervals during the experiment. For EEG recordings and power spectra, three gold-plated screws were implanted into the skull. In metaphit-treated animals, EEGs appeared as polyspikes and spike-wave complexes while the power spectra were increasing for 30-h period. The incidence and severity of metaphit-induced audiogenic seizures reached peak value 7-12 h after the injection. Both DSIP and DSIP(1-4) significantly increased power spectra of delta waves and decreased incidence of seizures, mean seizure grade and tonic component of metaphit-induced convulsions. Taken together, these results suggest that DSIP and its analogue DSIP(1-4) should be considered as potential antiepileptics.
Insights
Delta sleep-inducing peptide (DSIP) and its analogue DSIP(1-4) show promise as antiepileptics. These compounds reduced the severity and incidence of metaphit-induced audiogenic seizures in rats.
Area of Science:
- Neuroscience
- Pharmacology
- Epilepsy Research
Background:
- Audiogenic seizures are a model for studying epilepsy.
- Metaphit is a known inducer of audiogenic seizures.
- Delta sleep-inducing peptide (DSIP) is a naturally occurring peptide with potential neuromodulatory effects.
Purpose of the Study:
- To investigate the anticonvulsant effects of DSIP and its analogue DSIP(1-4) on metaphit-induced audiogenic seizures.
- To evaluate the impact of DSIP and DSIP(1-4) on electroencephalogram (EEG) power spectra, particularly delta waves.
Main Methods:
- Adult male Wistar rats were divided into five groups: saline, metaphit, DSIP, metaphit+DSIP, and metaphit+DSIP(1-4).
- Audiogenic seizures were induced using an electric bell (100+/-3 dB, 5-8 kHz).
- EEG recordings and power spectral analysis were performed after skull implantation of electrodes.
Main Results:
- Metaphit administration led to polyspike and spike-wave complex patterns in EEG and increased power spectra for 30 hours.
- The incidence and severity of metaphit-induced seizures peaked 7-12 hours post-injection.
- Both DSIP and DSIP(1-4) significantly increased delta wave power spectra and reduced seizure incidence, severity, and the tonic component of convulsions.
Conclusions:
- DSIP and its analogue DSIP(1-4) exhibit significant anticonvulsant properties against metaphit-induced audiogenic seizures.
- These findings suggest that DSIP and DSIP(1-4) hold potential as novel antiepileptic agents.
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