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Updated: Aug 29, 2026
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
Galactonate determination in urine by stable isotope dilution gas chromatography-mass spectrometry
Peter Schadewaldt1, Hans-Werner Hammen, Sara Stolpmann
1Klinik für Allgemeine Pädiatrie, Universitätsklinikum, Heinrich-Heine-Universität Düsseldorf, Metabolic Unit, Moorenstrasse 5, D-40225 Düsseldorf, Germany. schadewa@uni-duesseldorf.de
Abstract:
A stable isotope dilution assay was developed for the sensitive determination of D-galactonic acid. D-[U-13C(6)]galactono-1,4-lactone was prepared as internal standard. Unlabelled and U-13C-labelled D-galactonic acid species were converted to the N-(1-butyl)galactonamide pentaacetate derivatives and assessed by gas chromatography-mass spectrometry (GC-MS). Positive chemical ionisation and monitoring of the [MH-60](+)-ions in the galactonate chromatographic peak at m/z 402 and m/z 408 were used for quantification. The procedure was applied to study the variability of D-galactonate excretion in healthy subjects and galactosemic patients and to monitor the D-galactonate-D-galactitol ratio in human urine.
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