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Related Experiment Videos

[A new membrane-permeable peptide from human].

Tao Peng, Chun-lei Yang, Jing Xiao

    Hang Tian Yi Xue Yu Yi Xue Gong Cheng = Space Medicine & Medical Engineering
    |February 3, 2004
    PubMed
    Summary
    This summary is machine-generated.

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    The human Circadian locomotor output cycles kaput protein's DNA-binding peptide (hCLOCK's DNA_BIND) can cross cell membranes and enter the nucleus. This suggests potential for safe intracellular drug delivery.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Context:

    • Cellular uptake and translocation are crucial for drug delivery.
    • Understanding protein domain membrane permeability is key to developing new therapeutic strategies.
    • The hCLOCK DNA-binding peptide is a novel candidate for investigating these processes.

    Purpose:

    • To investigate the cell membrane translocation capability of the human hCLOCK DNA-binding peptide (hCLOCK's DNA_BIND).
    • To assess the peptide's distribution and accumulation within cells.
    • To evaluate factors influencing cellular uptake, such as time, concentration, and temperature.

    Summary:

    • Chemically synthesized and FITC-labeled hCLOCK's DNA_BIND was incubated with endothelial (ECV-304) and neuroglial cells.

    Related Experiment Videos

  • Fluorescence microscopy revealed that hCLOCK's DNA_BIND translocates across the cell membrane and accumulates in the nucleus.
  • Cellular uptake increased with incubation time and concentration, and was efficient at both 4°C and 37°C.

    • Impact:

    • Demonstrates the intrinsic cell membrane translocation ability of hCLOCK's DNA_BIND.
    • Highlights the peptide's potential as a safe and effective carrier for intracellular treatments.
    • Opens avenues for novel drug delivery systems targeting intracellular components.