Heat shock proteins expression in brain stem after subarachnoid hemorrhage in rats

The pathogenesis of brain damage after subarachnoid hemorrhage (SAH) especially at molecular or gene level remains unclear. We used complimentary deoxyribonucleic acid (cDNA) macroarray technique and compared gene expression in brain stem after experimental SAH in rats. The upregulation of several heat shock proteins (HSPs) demonstrated by cDNA array was further confirmed by Western blotting. The expressions of 9 genes were upregulated 30 minutes or 2 days after SAH. They included four upregulated HSPs: HSP90alpha, HSP60, HSP27, and HSP10. Western blotting demonstrated increases in the HSP27 and HSP10 proteins on Day 2. SAH enhanced the induction of several HSP mRNAs in the brainstems, even though the functions of these HSPs after SAH remain unclear.