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Updated: Aug 29, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Twin column chromatography for industrial-scale decontamination processes
1Forschungszentrum Jülich, Institut für Sicherheitsforschung und Reaktortechnik, P.O. Box 1913, D-52425 Jülich, Germany. u.wenzel@fz-juelich.de
Abstract:
A frontal chromatographic unit was devised consisting of a column-detector-column array. The unit is either equipped with identical columns (identical twins) or with columns of varying length (fraternal twins). Due to the finite nature of the columns, a prerun is formed at the column walls following the same regularities as the main stream. These regularities are used for the identification of the process termination below the detection limits of the monitor. For the implementation, a clear preference is given to the employment of fraternal twins, as the feed assay can be integrated into the separation process.
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