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[Relationship between programmed cell death mechanisms and neuronal necrosis induced by seizures]
Ren-zhe An1, Yong-ri Yin, Chun-ji Jin
1Department of Pediatrics, The Hospital Affiliated to The College of Medicine, Yanbian University, Yanji 133000, China.
Objective:
To clarify if programmed cell death mechanisms induced by seizures take part in the necrotic process of neurons.
Methods:
Seizure was induced by pilocarpine (P) in Sprague-Dawley adult rats which were allowed to recover for 24 or 72 hours before perfusion-fixation. Neuronal death was assessed by light microscopy with the hematoxylin-eosin (HE) staining and with in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL). Bax and Bcl-2 protein expression were examined by histochemistry.
Results:
Twenty-four and 72 hours after seizures, neuronal death in hippocampus CA1 region was morphologically necrotic. TUNEL-positive and morphologically necrotic cells increased in the hippocampal CA1 region at 72 hours after seizures, there was significant difference compared with controls (P < 0.001). Bax expression was also increased in the hippocampal CA1 region at 72 hours after seizures (P < 0.001), but Bcl-2 expression did not increase, while Bcl-2/Bax ratio decreased.
Conclusion:
Seizures induced late-onset neuronal necrosis was accompanied by programmed cell death mechanisms.