Related Experiment Video
Updated: Aug 29, 2026

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
Published on: October 3, 2018
Primitive hematopoietic stem cells shows a polyclonal pattern in myelodysplastic syndromes
Francesca Guidetti1, Sandra Grazioli, Francesca Capelli
1Department of Medical Science, University of Piemonte Orientale Amedeo Avogadro Novara, Italy.
Background And Objectives:
Clonal hematopoiesis is the hallmark of myelodysplastic syndromes, but the role played by pluripotent stem cells and progenitor cells in these disorders remains unclear.
Design And Methods:
Eight female patients with myelodysplastic syndrome were studied. X-chromosome inactivation patterns were analyzed in peripheral blood granulocytes, T-lymphocytes, single colonies originating from bone marrow progenitors and pluripotent stem cells, using the human androgen receptor locus polymorphism assay.
Results:
Granulocytes and progenitor cells were monoclonal in 7/8 cases. Immature stem cells showed a non-clonal pattern of X-inactivation and were detectable at diagnosis in the presence of clonal hematopoiesis. T-lymphocyte clonality was heterogeneous.
Interpretation And Conclusions:
In myelodysplastic syndromes, hematopoiesis may be dominated by a neoplastic clone by virtue of its biological advantage over a residual polyclonal, probably still normal, population of immature stem cells still able to grow in vitro.
More Related Videos
10:25Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
09:01Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Related Concept Videos
Multipotency of Hematopoietic Stem Cells
Hematopoiesis
Regulation of Hematopoietic Stem Cells
Production of Formed Elements
Most HSCs commit to...
Lineage Commitment
Differentiation of Common Myeloid Progenitor Cells