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Updated: Aug 29, 2026

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
Published on: December 30, 2021
'Agglutination and flocculation' of stem cells collected by apheresis due to cryofibrinogen
M A Siegenthaler1, D-H Vu, M Ebnöther
1Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.
Abstract:
Collection of peripheral stem cells by apheresis is a well-described process. Here, investigations concerning 'agglutination and flocculation' of stem cells collected from two patients are described. In both cases, cryoproteins were observed and cryofibrinogen was identified using high-resolution two-dimensional electrophoresis. In one case, peripheral stem cells were collected after a second course of mobilization, and the cells were immediately washed at 37 degrees C before being frozen, allowing their use, despite the presence of cryofibrinogen. In the other case, 'agglutination' was reversed by warming the bag, and plasma was removed before freezing.
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