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Related Experiment Videos

Microbioreactor arrays with parametric control for high-throughput experimentation.

Michel M Maharbiz1, William J Holtz, Roger T Howe

  • 1Berkeley Sensor & Actuator Center (BSAC), Dept. of Electrical Engineering and Computer Science, 497 Cory Hall, University of California at Berkeley, Berkeley, California 94720, USA.

Biotechnology and Bioengineering
|February 3, 2004
PubMed
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A novel microbioreactor array system enables precise control over high-throughput cell cultivations. This printed circuit board (PCB) based technology offers scalable, automated monitoring and gas delivery for optimized cell growth experiments.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Microfluidics

Background:

  • High-throughput cell cultivation requires precise control over environmental parameters.
  • Existing methods for controlling microscale cultures can be complex and difficult to scale.
  • Need for integrated systems for real-time monitoring and automated reagent delivery.

Purpose of the Study:

  • To demonstrate a scalable array technology for parametric control of high-throughput cell cultivations.
  • To integrate printed circuit board (PCB) technology with sensors and electrochemical gas generation for microbioreactor arrays.
  • To present results from an array of eight 250 microl microbioreactors with independent control.

Main Methods:

  • Utilized commercial printed circuit board (PCB) technology for array assembly.

Related Experiment Videos

  • Integrated off-the-shelf components including integrated circuit sensors and ISFET chips.
  • Developed an electrochemical gas generation system for precise oxygen and carbon dioxide dosing.
  • Implemented closed-loop control for temperature and continuous optical density monitoring.
  • Cultured Escherichia coli under varying microaerobic conditions.

    • Main Results:

    • Demonstrated independent parametric control (temperature, gas composition, pH) in an eight-bioreactor array.
    • Achieved reproducible gas delivery using electrochemical methods for microaerobic conditions.
    • Successfully monitored Escherichia coli growth and pH using integrated sensors.
    • Presented data on electrochemically generated oxygen for microaerobic control and carbon dioxide for pH dosing.

    Conclusions:

    • The developed PCB-based microbioreactor array technology offers a scalable solution for high-throughput cell cultivation.
    • Electrochemical gas generation provides a powerful and reproducible method for microreactor gas delivery.
    • The integrated system enables precise control and real-time monitoring, facilitating optimized cell growth studies.