Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Profiling ethylene-regulated gene expression in Arabidopsis thaliana by microarray analysis.

Guang Yan Zhong1, Guang Van Zhong, Jacqueline K Burns

  • 1Horticultural Sciences Department, University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.

Plant Molecular Biology
|February 6, 2004
PubMed
Summary

Ethylene significantly impacts gene expression in Arabidopsis thaliana leaves, regulating approximately 7% of genes. This study identifies key ethylene-regulated genes involved in biosynthesis, signaling, and various metabolic pathways, revealing complex hormonal crosstalk.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modification of carotenoid levels by abscission agents and expression of carotenoid biosynthetic genes in 'valencia' sweet orange.

Journal of agricultural and food chemistry·2013
Same author

Gene expression in Citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit.

Journal of experimental botany·2012
Same author

Light controls phospholipase A2alpha and beta gene expression in Citrus sinensis.

Journal of experimental botany·2010
Same author

Response of sweet orange (Citrus sinensis) to 'Candidatus Liberibacter asiaticus' infection: microscopy and microarray analyses.

Phytopathology·2008
Same author

CsPLDalpha1 and CsPLDgamma1 are differentially induced during leaf and fruit abscission and diurnally regulated in Citrus sinensis.

Journal of experimental botany·2008
Same author

A citrus abscission agent induces anoxia- and senescence-related gene expression in Arabidopsis.

Journal of experimental botany·2007

Area of Science:

  • Plant Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Ethylene is a crucial plant hormone regulating diverse developmental processes and stress responses.
  • Understanding ethylene's regulatory network at the transcriptomic level is vital for plant science.

Purpose of the Study:

  • To comprehensively analyze ethylene-regulated gene expression in Arabidopsis thaliana leaves using a large-scale microarray.
  • To identify specific genes and pathways modulated by ethylene, including its own signaling components and interactions with other pathways.

Main Methods:

  • Utilized an expressed sequence tag-based microarray encompassing approximately 6000 unique genes.
  • Compared gene expression profiles across wild-type plants (treated and untreated) and ethylene signaling mutants (etr1-1 and ctr1-1).

Related Experiment Videos

Main Results:

  • Identified approximately 7% of genes as ethylene-regulated, with distinct patterns observed between mutants and treatments.
  • Found that ethylene regulates genes in its own biosynthesis and signal transduction pathways, including numerous transcription factors and signaling components.
  • Observed down-regulation of chloroplast and photosynthetic genes, differential regulation of defense/PR genes, and links to sugar, auxin, wounding, and jasmonic acid signaling.

Conclusions:

  • Ethylene exerts broad control over gene expression in Arabidopsis leaves, influencing primary metabolism, development, and defense.
  • The study highlights significant crosstalk between ethylene signaling and other hormonal pathways, suggesting integrated regulatory networks.
  • Despite identifying many regulated genes, the functional roles of numerous ethylene-responsive genes remain to be elucidated.