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Updated: Aug 2, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells
Published on: May 13, 2010
Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells
Aleksandra Muratovska1, Michael R Eccles
1MRC-Dunn Human Nutrition Unit, Wellcome Trust/MRC building, Hills Rd, Cambridge CB2 2XY, UK.
Abstract:
The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase, or green fluorescent protein (GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm. These MPP-siRNAs efficiently reduced transient and stable expression of reporter transgenes in several mammalian cell types in a high proportion of cells, and demonstrated equivalent or better delivery characteristics than cationic liposomes with fewer manipulations.
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