Related Experiment Video
Updated: Aug 29, 2026
Use of a Hanging-weight System for Liver Ischemia in Mice
Published on: August 7, 2012
The hydroxyl radical scavenger MCI-186 protects the liver from experimental cold ischaemia-reperfusion injury
M Ninomiya1, M Shimada, N Harada
1Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan. nnmym@surg2.med.kyushu-u.ac.jp
Background:
Oxidative stress contributes to hepatic ischaemia-reperfusion (IR) injury in a biphasic pattern. In addition to direct cytotoxic effects, oxidative stress also initiates the signal transduction processes that promote second-phase liver injury. The present study investigated the effects of the hydroxyl radical scavenger MCI-186 on the biphasic process of hepatic cold IR injury.
Methods:
After cold preservation for 16 h, rat livers were reperfused on an isolated liver perfusion system for 120 min with oxygenated Krebs-Henseleit bicarbonate buffer. Perfusate samples were obtained serially, and portal flow rates were also recorded. To determine whether MCI-186 affected cytokine levels that control the second-phase injury, levels of interleukin (IL) 10 and tumour necrosis factor (TNF) alpha were measured in the perfusate.
Results:
Addition of MCI-186 1 mg/l into the perfusate significantly improved portal flow (P<0.050), hepatic enzyme release into the perfusate (P=0.038), total bile production (P=0.029) and malondialdehyde concentration (P=0.038). Furthermore, treatment with MCI-186 led to a substantial increase in IL-10 release (P=0.032). TNF-alpha levels were not affected.
Conclusions:
MCI-186, an agent ready for clinical use, appears to have direct and indirect protective effects against hepatic cold IR injury.
