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Quantitation of Endothelial Cell Adhesiveness In Vitro
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Influence of ionic and non-ionic radiographic contrast media on leukocyte adhesion molecules

Guy L J Vermeiren1, Roel Willems, Marc J Claeys

  • 1Department of Intensive Care, University Hospital of Antwerp, Wilrijkstraat 10, B 2650 Edegem, Belgium.

Mediators of Inflammation
|February 6, 2004
PubMed

Insights

Ionic radiographic contrast media (RCM) reduced granulocyte adhesion in vivo during coronary angiography, unlike non-ionic RCM. This contrasts with in vitro findings, highlighting RCM

Area of Science:

  • Cardiovascular Research
  • Immunology
  • Medical Imaging

Background:

  • Granulocytes play a key role in ischemia-reperfusion injury.
  • Previous studies on coronary angiography often overlook the impact of radiographic contrast media (RCM) on granulocyte function.
  • Understanding RCM effects is crucial for accurate leukocyte analysis during procedures.

Purpose of the Study:

  • To evaluate the in vivo effect of ionic versus non-ionic RCM on granulocyte adhesion during coronary angiography.
  • To investigate the in vitro influence of RCM ionicity and osmolarity on granulocyte adhesion molecules.

Main Methods:

  • A randomized patient study (n=37) assessed granulocyte adhesion following ionic and non-ionic RCM administration.
  • In vitro experiments examined the effects of different RCM properties on granulocyte adhesion molecules (CD11b expression).
  • Serum osmolarity and CD11b expression (mean fluorescence intensity) were measured.

Main Results:

  • Patient serum osmolarity increased significantly after RCM infusion (p < 0.05).
  • Non-ionic RCM significantly upregulated CD11b expression in vivo (p < 0.05).
  • Ionic RCM showed reduced in vivo CD11b expression but significantly decreased it in vitro (p < 0.05).

Conclusions:

  • Ionic RCM demonstrated a significant reduction in granulocyte adhesion molecule upregulation in vivo.
  • Ionic RCM exhibited opposing effects in vitro, decreasing CD11b expression.
  • The distinct in vivo and in vitro effects of RCM on leukocytes necessitate careful consideration during functional analysis.
Abstract

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