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Updated: Aug 29, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
Published on: September 24, 2015
Quantitative analysis of nucleic acids--the last few years of progress
Chunming Ding1, Charles R Cantor
1Bioinformatics Program and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA. cmding@bu.edu
Abstract:
DNA and RNA quantifications are widely used in biological and biomedical research. In the last ten years, many technologies have been developed to enable automated and high-throughput analyses. In this review, we first give a brief overview of how DNA and RNA quantifications are carried out. Then, five technologies (microarrays, SAGE, differential display, real time PCR and real competitive PCR) are introduced, with an emphasis on how these technologies can be applied and what their limitations are. The technologies are also evaluated in terms of a few key aspects of nucleic acids quantification such as accuracy, sensitivity, specificity, cost and throughput.
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