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[Study on mitochondrial DNA damage in peripheral blood nucleate cells of the workers exposed to acrylonitrile]
Sheng Ding1, Lai-ji Ma, Wei Fan
1Department of occupational health, Jinshan hospital, Fudan university, Shanghai 200540, China.
Objective:
To study the potential aging effect on workers exposed to acrylonitrile (ACN).
Methods:
The deletion rates of mitochondrial DNA (mtDNA) in peripheral blood nucleate cells of 47 exposed workers and 47 non-exposed workers (as control), as well as 12 old people and 12 young people were measured with polymerase chain reaction (PCR).
Results:
The positive rates of mtDNA deletion in peripheral blood nucleate cells were 17.02% in the workers exposed to ACN and 25.00% in group of old people. However, the mtDNA deletion was not detected in the control group and young people.
Conclusions:
ACN could induce mtDNA deletion in peripheral blood nucleate cells of the exposed workers. There may be a potential molecular effect of occupational ACN exposure on workers' aging.
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