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Updated: Aug 29, 2026
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
Published on: June 24, 2008
[Isolation and characterization of human placenta derived adherent cells and their hematopoietic growth factor
Jin He1, Yi Zhang, Xiao-xia Jiang
1Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Objective:
To isolate and culture human placenta derived adherent cells (hPDAC) and assay their hematopoietic growth factor expression.
Methods:
By enzyme digestion, hPDAC were isolated from human placenta tissue and cultured, and their biological characteristics were studied. The hematopoietic growth factor (HGF) mRNA expression of hPDAC was assayed by RT-PCR.
Results:
hPDAC was successfully isolated from human placenta tissue, which was further confirmed as mesenchymal stem cell-like cells. HGF including SCF, FL, G-CSF, GM-CSF, M-CSF and IL-6 were expressed in hPDAC.
Conclusion:
hPDAC could be used as feeder layer for umbilical cord blood CD(34)(+) cells ex vivo expansion.
