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Updated: Aug 29, 2026
In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
Published on: November 23, 2015
[In vitro amplification and identification of immature dendritic cells from murine bone marrow]
1Institute of Burn Research, Southwestern Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China.
Objective:
To establish a method for in vitro amplification of immature dendritic cells from murine bone marrow, and to identify it with morphological, immunological phenotype determination, and functional examination.
Methods:
Dendritic cells from murine bone marrow were cultured with different dosage of rmGM-CSF. The suspending cells were examined with scanning electronic microscope, and the non-sensitized T lymphocyte proliferation was observed by mixed lymphocyte reaction.
Results:
Dendritic cells (DC) cultured in lower dosage of rmGM-CSF (Gm(low)DC) exhibited typical characteristics of DCs with high expression of CD11c and low expression of CD40 and I-A/I-E, and non-expression of B7-1 on the surface of the cells. The capacity of Gm(low)DC to stimulate the proliferation of non-sensitized T lymphocyte in vitro was weaker than that of Gm(high)DC.
Conclusion:
Gm(low)DC exhibited typical characteristics of DC, immature in cell phenotype and cell functions, suggesting that our methods of immature DCs culturing was feasible. The dosage of rm GM-CSF has direct relationship with the maturation degree of DC. Generally speaking, mature DC was mainly induced by high dosage of rmGM-CSF, while immature DC by low dosage.
