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Published on: October 25, 2018
[Human antibodies to cedar pollen secreted by human peripheral lymphocytes transformed by Epstein-Barr virus]
Hiroshi Shinmoto1, Toshihiro Takahashi, Michie Shinmoto
1National Food Research Institute, Tokyo, Japan. shinmoto@affrc.go.jp
To establish immortalized human B-cells secreting antibodies to cedar pollen allergens, peripheral blood lymphocytes from 13 donors were transformed with Epstein-Barr virus. Of 5000 micro culture wells with transformed cell growth, supernatants from 88 wells were found to contain antibodies to pollen allergens. Fourteen supernatants reacted with a cedar allergen Cry j1 and 10 reacted with Cry j2. IgM class antibodies were predominant.
To establish immortalized human B-cells secreting antibodies to cedar pollen allergens, peripheral blood lymphocytes from 13 donors were transformed with Epstein-Barr virus. Of 5000 micro culture wells with transformed cell growth, supernatants from 88 wells were found to contain antibodies to pollen allergens. Fourteen supernatants reacted with a cedar allergen Cry j1 and 10 reacted with Cry j2. IgM class antibodies were predominant.
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