Prolactin induces adrenal hypertrophy

Although adrenocorticotropic hormone is generally considered to play a major role in the regulation of adrenal glucocorticoid secretion, several reports have suggested that other pituitary hormones (e.g., prolactin) also play a significant role in the regulation of adrenal function. The aim of the present study was to measure the adrenocortical cell area and to determine the effects of the transition from the prepubertal to the postpubertal period on the hyperprolactinemic state induced by domperidone (4.0 mg kg-1 day-1, sc). In hyperprolactinemic adult and young rats, the adrenals were heavier, as determined at necropsy, than in the respective controls: adults (30 days: 0.16 +/- 0.008 and 0.11 +/- 0.007; 46 days: 0.17 +/- 0.006 and 0.12 +/- 0.008, and 61 days: 0.17 +/- 0.008 and 0.10 +/- 0.004 mg for treated and control animals, respectively; P < 0.05), and young rats (30 days: 0.19 +/- 0.003 and 0.16 +/- 0.007, and 60 days: 0.16 +/- 0.006 and 0.13 +/- 0.009 mg; P < 0.05). We selected randomly a circular area in which we counted the nuclei of adrenocortical cells. The area of zona fasciculata cells was increased in hyperprolactinemic adult and young rats compared to controls: adults: (61 days: 524.90 +/- 47.85 and 244.84 +/- 9.03 microm2 for treated and control animals, respectively; P < 0.05), and young rats: (15 days: 462.30 +/- 16.24 and 414.28 +/- 18.19; 60 days: 640.51 +/- 12.91 and 480.24 +/- 22.79 microm2 ; P < 0.05). Based on these data we conclude that the increase in adrenal weight observed in the hyperprolactinemic animals may be due to prolactin-induced adrenocortical cell hypertrophy.