Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitotracker green is a P-glycoprotein substrate.

Luis F Marques-Santos1, José G P Oliveira, Raquel C Maia

  • 1Laboratório de Imunologia Tumoral, Departamento de Bioquímica Médica--ICB, Universidade Federal do Rio de Janeiro,Brazil. lfmarques@bioqmed.ufrj.br

Bioscience Reports
|February 7, 2004
PubMed
Summary

Mitotracker Green (MTG), a mitochondrial probe, is a substrate for P-glycoprotein, a multidrug resistance protein. Its fluorescence is affected by P-glycoprotein activity, impacting mitochondrial studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crosstalk between biological and chemical diversity with cytotoxic and cytostatic effects of Aphanothece halophytica in vitro.

Anais da Academia Brasileira de Ciencias·2022
Same author

Detachment of Hexokinase II From Mitochondria Promotes Collateral Sensitivity in Multidrug Resistant Chronic Myeloid Leukemia Cells.

Frontiers in oncology·2022
Same author

Low rate of relapse after twelve-dose multidrug therapy for hansen's disease: A 20-year cohort study in a brazilian reference center.

PLoS neglected tropical diseases·2021
Same author

Synthesis of new α-Aryl-α-tetralones and α-Fluoro-α-aryl-α-tetralones, preliminary antiproliferative evaluation on drug resistant cell lines and in silico prediction of ADMETox properties.

Bioorganic chemistry·2021
Same author

Selective Cytotoxicity of Piperine Over Multidrug Resistance Leukemic Cells.

Molecules (Basel, Switzerland)·2021
Same author

Expression of nuclear XIAP associates with cell growth and drug resistance and confers poor prognosis in breast cancer.

Biochimica et biophysica acta. Molecular cell research·2020

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • P-glycoprotein (P-gp) is crucial in multidrug resistance (MDR) and expressed in normal tissues.
  • Flow cytometry and confocal microscopy enable new fluorescent dye applications.
  • MDR protein activity is often overlooked in cellular studies using fluorescent probes.

Purpose of the Study:

  • To investigate if Mitotracker Green (MTG) is a substrate for P-glycoprotein.
  • To determine the impact of P-glycoprotein activity on MTG fluorescence.
  • To assess the influence of MDR modulators on MTG staining in resistant cells.

Main Methods:

  • Flow cytometry was used to analyze MTG fluorescence in a human erythroleukemic cell line and its resistant counterpart.
  • In vitro studies involved MDR modulators like Cyclosporin A, Verapamil, and Trifluoperazine.

Related Experiment Videos

Main Results:

  • Mitotracker Green (MTG) was identified as a P-glycoprotein substrate.
  • MTG fluorescence profile is dependent on P-glycoprotein activity.
  • MDR modulators significantly altered MTG fluorescence in the resistant cell line.

Conclusions:

  • The evaluation of mitochondrial properties using MTG requires consideration of P-glycoprotein expression and activity.
  • MTG's utility as a mitochondrial probe may be confounded by P-gp-mediated efflux.
  • Further research is needed to validate MTG use in P-gp expressing cells.