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Interaction of ibuprofen with eukaryotic membrane lipids
Henning Lygre1, Grete Moe, Holm Holmsen
1Department of Odontology-Dental Pharmacology, University of Bergen, Bergen, Norway. henning.lygre@odont.uib.no
Acta Odontologica Scandinavica
|February 7, 2004
Summary
Ibuprofen interacts with model cell membranes, affecting lipid properties. This study shows how the drug interacts with various membrane lipids, impacting their phase transitions and melting temperatures.
Area of Science:
- Biochemistry
- Molecular Biology
- Pharmacology
Background:
- Amphiphilic drugs possess versatile molecular mechanisms.
- Eukaryotic cell membranes are composed of various lipids, including phosphatidylcholines (PC), phosphatidylserines (PS), and phosphatidylethanolamines (PE).
Purpose of the Study:
- To investigate the interaction of ibuprofen with model eukaryotic membrane lipids.
- To determine the effect of ibuprofen on the biophysical properties of lipid monolayers and liposomes.
Main Methods:
- Langmuir technique to determine pressure/molecular area isotherms of glycerophospholipids monolayers at 37°C.
- Differential scanning calorimetry (DSC) to analyze phase transition parameters in liposomes.
Main Results:
- Ibuprofen interacted with saturated phosphatidylcholine (PC) monolayers in a concentration-independent manner.
- Ibuprofen interacted with liposomes composed of saturated and unsaturated phosphatidylcholines (PC), phosphatidylserines (PS), and saturated phosphatidylethanolamines (PE).
- A decrease in lipid melting temperature (Tm) and altered enthalpy (ΔH) of phase transitions were observed in liposomes upon ibuprofen interaction.
Conclusions:
- Ibuprofen interacts with various components of model eukaryotic cell membranes.
- The interaction of ibuprofen with membrane lipids alters their phase transition properties, potentially influencing membrane function.