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Related Experiment Videos

Asthma genetics 2003.

Scott T Weiss1, Benjamin A Raby

  • 1Channing Laboratory, Brigham and Women's Hospital, 181 Longwood Avenue, Boston, MA 02115, USA. scott.weiss@channing.harvard.edu

Human Molecular Genetics
|February 7, 2004
PubMed
Summary
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Positional cloning identified two new asthma susceptibility genes, PHF11 and DPP10, advancing allergy research. This method, using genetic association, also provided mixed results for the known asthma gene ADAM33.

Area of Science:

  • Genetics
  • Genomics
  • Molecular Biology

Background:

  • Positional cloning is a key method for identifying genes associated with complex traits.
  • The completion of the human genome project has accelerated this approach.
  • Asthma and allergy research benefits from identifying genetic susceptibility factors.

Purpose of the Study:

  • To identify novel genes contributing to asthma susceptibility using positional cloning.
  • To further investigate the role of ADAM33 in asthma pathogenesis.
  • To evaluate the effectiveness and limitations of positional cloning for complex trait gene discovery.

Main Methods:

  • Collection of well-phenotyped human cohorts (family-based and case-control).
  • Generation of high-density single-nucleotide polymorphism linkage disequilibrium maps.

Related Experiment Videos

  • Application of statistical methods to detect genetic associations with asthma.

    • Main Results:

    • Identification of two novel asthma susceptibility genes: PHF11 and DPP10.
    • PHF11 and DPP10 were not previously linked to asthma or allergy pathobiology.
    • Further, albeit mixed, evidence was found supporting ADAM33 as an asthma susceptibility gene.

    Conclusions:

    • Positional cloning is a powerful tool for discovering complex trait genes, including those for asthma.
    • The identification of PHF11 and DPP10 opens new research avenues in asthma and allergy.
    • The study highlights both the strengths and limitations of positional cloning in genetic research.