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Heat shock factor regulates VDUP1 gene expression.
Kun-Yong Kim1, Sun Mi Shin, Jae Kwang Kim
1Laboratory of Immunology, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon 305-333, Republic of Korea.
Biochemical and Biophysical Research Communications
|February 10, 2004
Summary
Heat shock factor (HSF) regulates vitamin D3 up-regulated protein 1 (VDUP1) expression under cellular stress. This study identifies the heat shock factor element (HSE) as crucial for VDUP1 induction by high density and serum deprivation in Bosc cells.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Vitamin D3 up-regulated protein 1 (VDUP1) interacts with thioredoxin (TRX) and acts as its antagonist.
- VDUP1 expression is modulated by cellular stresses like ROS, UV, and heat shock.
Purpose of the Study:
- To investigate the transcriptional regulation of VDUP1 expression induced by cellular stresses.
- To identify the specific transcription factors and cis-elements involved in VDUP1 induction.
Main Methods:
- Cloning and reporter assays of the VDUP1 promoter region with deletions.
- Gel-shift and supershift assays to confirm transcription factor binding.
- Analysis of cis-elements within the VDUP1 promoter.
Main Results:
- An inducible expression of VDUP1 was observed in Bosc cells under high density and serum deprivation.
- The heat shock factor element (HSE) was identified as a key cis-element in the VDUP1 promoter.
- Deletion of HSE abolished stress-induced VDUP1 transcription, and heat shock factor (HSF) binding was confirmed.
- Enforced HSF expression or heat shock increased endogenous VDUP1 transcription.
Conclusions:
- Heat shock factor (HSF) is a critical transcription factor for the stress-induced up-regulation of VDUP1.
- The heat shock factor element (HSE) mediates the transcriptional response of VDUP1 to cellular stresses like high density and serum deprivation.