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Related Experiment Videos

Complex regulatory interactions control CRH gene expression.

Richard C Nicholson1, Bruce R King, Roger Smith

  • 1Mothers and Babies Research Centre, Hunter Medical Research Institute, University of Newcastle, NSW 2310, Australia. Rick.Nicholson@hunter.health.nsw.gov.au

Frontiers in Bioscience : a Journal and Virtual Library
|February 10, 2004
PubMed
Summary
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Researchers identified key elements regulating the corticotrophin-releasing hormone (CRH) gene. Glucocorticoids

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Gene Regulation

Background:

  • Glucocorticoids differentially regulate corticotrophin-releasing hormone (CRH) production in the hypothalamus and placenta.
  • Understanding the molecular mechanisms of CRH gene regulation is crucial for various physiological processes.

Purpose of the Study:

  • To identify key regulatory elements and transcription factors involved in CRH gene expression.
  • To elucidate the differential effects of glucocorticoids on CRH production.

Main Methods:

  • Analysis of the CRH promoter in mouse pituitary tumor-derived AtT20 cells as a hypothalamic model.
  • Electrophoretic mobility shift assays (EMSA) to identify transcription factor binding.
  • Identification of cAMP responsive elements (CRE) and novel response elements.

Related Experiment Videos

Main Results:

  • Two cAMP responsive elements (CRE and CDXRE) were identified in the CRH promoter.
  • Glucocorticoids inhibit cAMP-stimulated CRH production via a negative glucocorticoid response element (nGRE) acting on the CRE.
  • Tissue-specific transcription factors (CREB, Fos, cJun) bind to the CRE, with a novel CRE-binding factor identified in brain and placenta.

Conclusions:

  • CRH gene regulation involves distinct cis-regulatory elements and transcription factors.
  • Tissue-specific expression of transcription factors mediates differential CRH gene regulation by glucocorticoids.
  • A novel transcription factor contributes to CRH gene regulation in the brain and placenta.