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Novel method using a laser scanning cytometer for detection of mycobacteria in clinical samples
Cidália Pina-Vaz1, Sofia Costa-Oliveira, Acácio Gonçalves Rodrigues
1Department of Microbiology, Faculty of Medicine, University of Porto, Hospital S João, Porto, Portugal. micteam@clix.pt
Journal of Clinical Microbiology
|February 10, 2004
Abstract:
In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect MYCOBACTERIA: