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Chemiluminescent detection of alkaline phosphatase in PhastGel
N G Theodosiou1, C L Chatot, C A Ziomek
1Genzyme Corporation, Framingham, MA 01701.
Biotechniques
|December 1, 1992
Abstract:
A chemiluminescent assay has been applied to the detection of alkaline phosphatase on PhastGel containing lysates of preimplantation mouse embryos. The very sensitive detection capabilities reported for the chemiluminescent system led to the investigation of its applicability to the characterization of the alkaline phosphatases in one embryo or less and to compare the sensitivity of two different commercial alkaline phosphatase chemiluminescent assays to a colorimetric assay.