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Distribution and immunoregulatory properties of antisecretory factor
Todd S Davidson1, William F Hickey
11Department of Pathology, Borwell Building, Dartmouth Medical School -DHMC, Lebanon, NH 03756, USA.
Laboratory Investigation; a Journal of Technical Methods and Pathology
|February 10, 2004
Summary
Antisecretory factor (ASF) regulates immune responses. This protein, identified using TLD-1A8A antibodies, is expressed on immune cells and in lymphoid organs, suggesting a novel role in immune system regulation.
Area of Science:
- Neuroimmunology
- Immunology
Background:
- Inflammation in the central nervous system (CNS) involves diverse cells and molecules.
- Microglial cells are implicated in CNS inflammation control.
- Antisecretory factor (ASF) was previously known for inhibiting intestinal fluid secretion.
Purpose of the Study:
- To investigate the role of antisecretory factor (ASF) in immune reactions.
- To characterize the expression and function of ASF in the immune system.
Main Methods:
- Utilized TLD antibodies, specifically clone TLD-1A8A, targeting rat microglial antigens.
- Performed T-cell proliferation and mixed leukocyte response assays.
- Conducted flow cytometry, immunohistochemistry, and Western blot analyses.
Main Results:
- Administration of TLD-1A8A increased T-cell proliferation.
- ASF is expressed on macrophages, B cells, and dendritic cells, but not T cells or granulocytes.
- ASF is present in spleen, thymus, lymph nodes, Peyer's patch, CNS perivascular areas, and secondary lymphoid organs.
Conclusions:
- ASF plays a role in regulating immune reactions.
- ASF expression is widespread in immune cells and tissues.
- ASF may have a previously unrecognized function in immune system regulation.