Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Poly-beta-hydroxybutyrate in staphylococci.

E M Szewczyk1

  • 1Department of Pharmaceutical Microbiology, Medical University of Lódź, Poland.

FEMS Microbiology Reviews
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Staphylococcus cohnii hemolysins - isolation, purification and properties.

Folia microbiologica·2009
Same author

Plasmids of Staphylococcus cohnii isolated from the intensive-care unit.

Folia microbiologica·2004
Same author

Evaluation of selected features of Staphylococcus cohnii enabling colonization of humans.

Folia microbiologica·2002
Same author

Staphylococcus cohnii--resident of hospital environment: cell-surface features and resistance to antibiotics.

Acta microbiologica Polonica·2000
Same author

Predominant staphylococci in the intensive care unit of a paediatric hospital.

The Journal of hospital infection·2000
Same journal

Feeding the invaders: How metabolic imbalance shapes infection and biofilm development.

FEMS microbiology reviews·2026
Same journal

Interactions between extracellular vesicles and viruses: lessons learned across species and kingdoms.

FEMS microbiology reviews·2026
Same journal

Killer Peptide: an antibody-derived self-assembling peptide bridging antimicrobial and host-defense mechanisms.

FEMS microbiology reviews·2026
Same journal

From gatekeeper to target: MAPK cascades as control circuits at the insect-microbe interface.

FEMS microbiology reviews·2026
Same journal

The role of fungal G protein-coupled receptors in interspecies cell-cell communication.

FEMS microbiology reviews·2026
Same journal

Convergent symbioses: morphology, life history, and niche specialization in coral and lichen mutualisms.

FEMS microbiology reviews·2026
See all related articles

Staphylococci bacteria store poly-beta-hydroxybutyrate (PHB) for metabolism. While lacking PHB depolymerase, these bacteria possess beta-hydroxybutyrate dehydrogenase, crucial for polymer breakdown.

Area of Science:

  • Microbiology
  • Biochemistry

Background:

  • Staphylococci are chemoorganotrophic bacteria commonly found in animal and human hosts.
  • These bacteria can accumulate poly-beta-hydroxybutyrate (PHB), an energy reserve polymer, within their cells.

Purpose of the Study:

  • To investigate the metabolism of poly-beta-hydroxybutyrate (PHB) in staphylococci.
  • To identify enzymes involved in PHB degradation within staphylococcal species.

Main Methods:

  • Enzyme assays were conducted to detect PHB depolymerase activity.
  • Beta-hydroxybutyrate dehydrogenase was estimated, purified, and characterized from staphylococcal extracts.

Main Results:

  • Poly-beta-hydroxybutyrate (PHB) serves as a substrate for endogenous metabolism in staphylococci, preceding the degradation of other cellular components like amino acids, proteins, and RNA.

Related Experiment Videos

  • No detectable poly-beta-hydroxybutyrate (PHB) depolymerase activity was found in the staphylococci studied.
  • Beta-hydroxybutyrate dehydrogenase was successfully identified, purified, and characterized, indicating its role in PHB metabolism.

    • Conclusions:

    • Staphylococci metabolize intracellular poly-beta-hydroxybutyrate (PHB) via beta-hydroxybutyrate dehydrogenase, not through a depolymerase enzyme.
    • The findings elucidate a specific pathway for poly-beta-hydroxybutyrate (PHB) catabolism in staphylococci, highlighting the importance of beta-hydroxybutyrate dehydrogenase.