Related Experiment Video
Updated: Aug 29, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Isolation and characterization of microorganisms capable of decolorizing various triphenylmethane dyes
Deepak Kumar Sharma1, Harvinder Singh Saini, Manjinder Singh
1Department of Microbiology, Guru Nanak Dev University, Amritsar-143005, India.
Abstract:
Various soil and sludge samples collected from the vicinity of textile dyeing industries and waste disposal sites were used for enrichment of microbial population in the presence of triphenylmethane (TPM) dye Acid Violet-17 (AV-17). Twenty-five (25) isolates were screened for their ability to decolorize AV-17 dye added at a rate of 10 mgl(-1) in mineral salts medium (MSM) agar plates. Five bacterial isolates belonging to Bacillus sp., Alcaligenes sp. and Aeromonas sp. were selected on the basis of their higher decolorization ability and were used to develop a bacterial consortium. The consortium was able to efficiently decolorize various TPM dyes viz. Acid Violet-17 (86%), Acid Blue-15 (85%), Crystal Violet (82%), Malachite Green (82%) and Brilliant Green (85%). The consortium will be further used for designing efficient and cost effective treatment system for effluents of textile processing industries (TPI).
More Related Videos
06:53Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
16:33Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
Related Concept Videos
Differential Staining Technique
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Methods to Assess Microbial Populations