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Related Experiment Videos

Hepatic dendritic cell subsets in the mouse.

Ieva Jomantaite1, Nektarios Dikopoulos, Andrea Kröger

  • 1Department of Medical Microbiology, University of Ulm, Ulm, Germany.

European Journal of Immunology
|February 10, 2004
PubMed
Summary

Mouse liver dendritic cells (DC) include two distinct subsets: B220(+) and B220(-). These subsets show different functions and responses to activation, impacting immune responses in the liver.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The non-parenchymal cells of the mouse liver contain various immune cells, including dendritic cells (DC).
  • Understanding DC heterogeneity is crucial for deciphering liver immune responses.

Purpose of the Study:

  • To characterize distinct subsets within the CD11c(+) dendritic cell population in the mouse liver.
  • To investigate the functional differences and responses to activation signals between these DC subsets.

Main Methods:

  • Flow cytometry was used to identify and quantify DC subsets based on markers like CD11c, B220, CD40, CD80, CD86, and MHC class II.
  • Experiments involved immunocompetent and immunodeficient [recombinase-activating gene-1 (RAG1)(-/-)] mice.
  • In vivo and in vitro stimulation models, including NKT cell activation and viral infection, were employed.

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  • T cell priming assays were conducted to assess DC function.

    • Main Results:

    • Two major hepatic CD11c(+) DC subsets were identified: B220(+) and B220(-).
    • The B220(-) subset consisted of immature and mature populations, while the B220(+) subset was characterized as 'plasmacytoid'.
    • B220(+) DC produced type I interferon upon stimulation, whereas B220(-) DC primed T cells.
    • NKT cell activation led to an increase in B220(-) DC and a decrease in B220(+) DC.
    • Viral infection induced maturation of both DC subsets.

    Conclusions:

    • The mouse liver contains at least two distinct DC subsets, B220(+) and B220(-), with unique marker profiles and functional capabilities.
    • These subsets exhibit differential responses to immune stimuli, suggesting specialized roles in liver immunity.
    • The findings highlight the complexity of hepatic DC populations and their contribution to immune regulation.