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In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Bacteriological investigation of infected root canals in Benin City, Nigeria
1Department of Restorative Dentistry, School of Dentistry, University of Benin, Nigeria.
Abstract:
Possible viable bacteria were isolated and determined through culture based on paper point inoculation from infected root canals of 50 patients who presented for endodontic therapy at the school of Dentistry, University of Benin Teaching Hospital and Central Hospital, Benin City. Eighty-one strains of bacteria were isolated from 50 patients. The bacterial isolates were Streptococcus species, (51), Klebsiella species (17), E. coll (5), Staphylococcus aureus (3), Micrococcus species (2), Neisseria spp. (2) and Lactobacillus. The isolation of Klebsiella spp from the study is particularly interesting, constituting 21% of all the isolated bacteria. The implications of these findings in root canal infections are presented.

